The key difference between CRISPR and CRISPR Cas9 is that CRISPR (clustered regularly interspaced short palindromic repeat) is a naturally occurring prokaryotic immune defence mechanism while CRISPR cas9 is an RNA-guided Cas9 nuclease which is a part of the CRISPR adaptive immune system. CRISPR is an antiviral defence immune system found in bacteria and archaea. […]
Difference Between CDS and cDNA
The key difference between CDS and cDNA is that CDS or coding sequence is the part of a transcript that is actually translated into protein while cDNA sequence is a DNA sequence derived from the mRNA by reverse transcription. A gene is a nucleotide sequence which codes for a protein. It consists of different regions […]
Difference Between Mutagen and Teratogen
The key difference between mutagen and teratogen is that mutagen is a substance or an agent that causes a change in the sequence of the DNA of an individual while a teratogen is an agent that causes harm to the fetus or the developing embryo during pregnancy. Mutagens and teratogens are usually chemical agents. However, […]
Difference Between DNA and RNA Nucleotide
The key difference between DNA and RNA nucleotide is that DNA nucleotide or deoxyribonucleotide contains deoxyribose sugar while RNA nucleotide or ribonucleotide contains ribose sugar. Nucleotides are the basic unit of nucleic acids. They are the building blocks or monomers of DNA and RNA. They link with each other to form a polynucleotide chain, which […]
Difference Between Consensus PCR and Pan PCR
The key difference between consensus PCR and pan PCR is that consensus PCR always targets conserved regions whereas Pan PCR targets variable regions to identify different strains of a group Polymerase chain reaction (PCR) is a gene amplification technique that consists of three main steps as denaturation, annealing and extension. This is widely used in […]
Difference Between Conserved and Consensus Sequence
The key difference between conserved and consensus sequence is that conserved sequence refers to similar sequences of nucleic acids or amino acids that occur in different or same species over generations while consensus sequence is a commonly encountered nucleotides sequence or amino acid sequence found in a highly conserved region of DNA or RNA or […]
Difference Between Radioactive and Nonradioactive Probes
The key difference between radioactive and nonradioactive probes is that radioactive probes are single-stranded DNA or RNA sequences that are labeled with radioactive isotopes while nonradioactive probes are single-stranded DNA or RNA sequences that are labeled with a chemical tag or a fluorescent tag. Nucleic acid hybridization is an important technique in molecular biology, especially […]
Difference Between Watson and Crick and Hoogsteen Base Pairing
The key difference between Watson and Crick and Hoogsteen base pairing is that Watson and Crick’s base pairing is the standard way that describes the formation of base pairs between purines and pyrimidines. Meanwhile, Hoogsteen base pairing is an alternative way of forming base pairs in which the purine takes a different conformation with respect […]
Difference Between Random Primers and Oligo dT
The key difference between random primers and oligo dT is that the random primer is a mixture of all possible hexamer oligonucleotide sequences, while the oligo dT primer consists of a single-stranded stretch of 12–18 deoxythymidines. Reverse transcription is a mechanism in which cDNA can be synthesized using mRNA or any species of RNA. In […]
Difference Between Regulatory and Repressor Protein
The key difference between regulatory and repressor protein is that regulatory protein can promote or inhibit the transcription of genes. Meanwhile, the repressor protein inhibits the expression of one or more genes. Before going further into the discussion of the difference between regulatory and repressor protein, let’s briefly discuss gene regulation. Gene is a specific […]
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