The key difference between DAPI and Hoechst is that DAPI is a dye that is useful in staining DNA content in fixed cells, while Hoechst is a dye that is useful in staining DNA content in live cells. Differential staining of the cell cycle is a very important method. It allows the staining of DNA […]

The key difference between sandwich Elisa and competitive Elisa is that sandwich ELISA is a type of ELISA commonly used to detect and quantify antigens in immunoassays, while competitive ELISA is a type of Elisa commonly used to detect and quantify antibodies in immunoassays. Enzyme-Linked Immunosorbent Assay (ELISA) is an immunoassay technique that detects diseases. […]

The key difference between post transcriptional and post translational modification is that post transcriptional modification occurs after the transcription step of the protein synthesis, while post translational modification occurs after the translation step of the protein synthesis. Protein synthesis contains two basic mechanisms as transcription and translation. In transcription, a DNA molecule is converted into […]

The key difference between native and denaturing gel electrophoresis is that in native gel electrophoresis, the biomolecule of interest is maintained its normal or native structure when running it through the gel, while in denaturing gel electrophoresis, the biomolecule of interest is not maintained its normal or native structure when running it through the gel. […]

The key difference between serum protein electrophoresis and immunofixation is that serum protein electrophoresis is a technique that examines proteins in the serum by only using electrophoresis, while serum protein immunofixation is a technique that examines proteins in the serum by using both electrophoresis and precipitation. Serum protein electrophoresis and immunofixation are laboratory techniques used […]

The key difference between T4 and Lambda phage is that T4 phage is a virulent phage that undergoes a lytic lifecycle, while Lambda phage is a temperate phage that undergoes a lysogenic lifecycle. Bacteriophages are bacteria infecting viruses. They infect and replicate only inside the host bacterium. Some phages undergo the lytic replicative cycle, while […]

The key difference between DNA dependent DNA polymerase and DNA dependent RNA polymerase is that DNA dependent DNA polymerase catalyzes the synthesis of a double-stranded DNA molecule during DNA replication while DNA dependent RNA polymerase catalyzes the synthesis of a single-stranded RNA molecule mainly during transcription. DNA is the genetic material of humans and of […]

The key difference between Taq and Pfu polymerase is that Taq polymerase possesses 5’-3’ exonuclease proofreading activity while Pfu polymerase possesses 3’-5’ exonuclease proofreading activity. DNA polymerase catalyzes the synthesis of DNA during replication. DNA polymerase utilizes a template DNA strand to synthesize the complementary DNA strand. In addition to its function as a polymerase, […]

The key difference between de novo methylation and maintenance methylation is that de novo methylation utilizes the enzymes DNMT3A and DNMT3B, while maintenance methylation utilizes one enzyme, DNMT1. DNA methylation is a process essential for transcriptional regulation, silencing of transposable elements, and genome stabilization. De novo methylation and maintenance methylation are two key components of […]

The key difference between type 1 2 and 3 restriction endonuclease is that the cleavage site of type 1 restriction endonuclease is random and is away from the recognition site, while the cleavage site of type 2 restriction endonuclease is at the recognition site and the cleavage site of type 3 restriction endonuclease is 20-30 […]