The key difference between 1D and 2D gel electrophoresis is the properties used for the separation of proteins on gel electrophoresis. 1D gel electrophoresis only separates proteins based on the molecular weight while 2D gel electrophoresis separates proteins based on its iso-electric point and molecular weight. Protein separation by gel electrophoresis is an important technique […]

The key difference between knock in and knockout relies on the method used to create a transgenic organism. In gene knock in, insertion of a new gene takes place while in gene knockout, removal of an existing gene takes place to create a transgenic organism. Transgenic organisms are organisms with an altered genetic constitution. ‘Recombinant […]

The key difference between gene amplification and gene cloning is that the gene amplification is the process of making many copies of the gene of interest in vitro by a polymerase chain reaction. But,  gene cloning is a process of making many copies of the gene of interest in vivo by making a recombinant plasmid […]

The key difference between nucleoside and nucleotide reverse transcriptase inhibitors is that nucleoside reverse transcriptase inhibitors should be phosphorylated by host’s cellular kinases while nucleotide reverse transcriptase inhibitors do not need to undergo initial phosphorylation. Reverse transcriptase is an enzyme that converts RNA molecule into ssDNA. HIV and retroviruses possess this enzyme to synthesize ssDNA […]

The key difference between shotgun sequencing and next generation sequencing is that shotgun sequencing is a sequencing method which randomly breaks up DNA sequences into many small fragments and reassembles the sequence by observing the overlapping regions while next Next Generation Sequencing (NGS) is an advanced method of genetic sequencing which depends on capillary electrophoresis. […]

The key difference between FISH and CGH is that FISH is a molecular technique that detects specific DNA sequence on a chromosome using fluorescently labeled probes, while CGH is another molecular cytogenetic technique that detects alterations in the genomic DNA. Cytogenetic analysis plays an important role in medicine when detecting chromosomal abnormalities such as aneuploidies, […]

The key difference between metagenomics and metatranscriptomics relies on the type of biomolecules studied in each area. Metagenomics studies DNA, its sequences and its behaviour in organisms, while metatranscriptomics studies the transcribed DNA, mainly mRNA sequences and its behaviour in organisms. Metagenomics and metatranscriptomics are two fields that have gained much popularity over the years […]

The key difference between clone by clone sequencing and shotgun sequencing lies in their method of conduct. Clone by clone sequencing method involves mapping of chromosomes and cloning prior to sequencing while clone by shotgun sequencing omits both chromosome mapping and cloning steps during the sequencing. Clone by clone sequencing and clone by shotgun sequencing […]

The key difference between DNA sequence mutations and epigenetic modifications is that during DNA sequence mutations, changes occur in the original DNA sequences while during epigenetic modifications, changes do not occur in the original DNA sequences. The genome represents the overall genetic information of an organism in the form of precise DNA sequences or genes. […]

The key difference between lincRNA and lncRNA is the length of the RNA. That is; the lincRNAs are long strands of RNA whereas lncRNAs are comparatively short strands of RNA. RNA or Ribonucleic acid is an important biomolecule which has varied functions. There are many new discoveries taking place in terms of RNA. lincRNA and […]