The key difference between homologous recombination and site-specific recombination is that in homologous recombination, genetic material is exchanged between two identical molecules of double-stranded or single-stranded nucleic acids such as DNA or RNA, whereas in site-specific recombination, DNA strand exchange takes place between DNA segments that possess at least a certain degree of sequence homology but no […]
Difference Between Chromosomal DNA and Extrachromosomal DNA
The key difference between chromosomal DNA and extrachromosomal DNA is that chromosomal DNA is genomic DNA that is crucial in the development, growth and reproduction of an organism, while extrachromosomal DNA is non-genomic DNA that is found off the chromosomes and nonessential for the development, growth and reproduction of an organism. The essential genetic information is passed on to […]
Difference Between PFGE and CRP Analysis
The key difference between PFGE and CRP analysis is that PFGE is a laboratory technique used for the separation of large DNA molecules by applying an electric field that periodically changes, while CRP (c-reactive protein) analysis is a technique that detects a ring-shaped protein in blood plasma. PFGE and CRP analysis are two techniques used by scientists to detect different […]
Difference Between LAMP and PCR Test
The key difference between LAMP and PCR test is that LAMP is conducted at constant temperature (60-650C) to produce copies of DNA through amplification, while PCR is conducted using a series of temperature changes to produce copies of DNA through amplification. LAMP and PCR are both in-vitro amplification techniques used to produce thousands of copies […]
Difference Between Linker and Adaptor
The key difference between linker and adaptor is that a linker does not have cohesive ends while an adaptor has one cohesive end. DNA ligation is the process of joining two DNA molecules together, forming phosphodiester bonds. The enzyme called DNA ligase catalyzes this reaction. It is one of the critical steps in modern molecular […]
Difference Between Substitution Insertion and Deletion Mutations
The key difference between substitution insertion and deletion mutations is their cause. Substitution mutations occur due to a substitution of a base pair from a different base pair, while insertion mutations occur due to the addition of extra nucleotides into a DNA sequence and deletion mutations occur due to the removing of one or more […]
Difference Between dATP and ddATP
The key difference between dATP and ddATP is that dATP does not terminate DNA synthesis while ddATP is able to terminate DNA synthesis. This is because ddATP has an H atom attached to 3ʹ position of the pentose sugar while dATP has an OH group attached to 3ʹ position. dNTPs are building blocks of DNA. […]
Difference Between dNTP and DdNTP
The key difference between dNTP and ddNTP is that dNTP or deoxyribonucleotides are the building blocks of DNA and have a 3ʹ-OH group on the pentose sugar structure while ddNTP or dideoxynucleoside triphosphates are nucleotides that lack 3ʹ-OH group and they are used in Sanger dideoxy DNA sequencing technique to produce different length DNA sequences. […]
Difference Between Electroporation and Microinjection
The key difference between electroporation and microinjection is that electroporation is a technique that uses a high voltage electric pulse to deliver DNA into host cells while microinjection is a technique that uses a fine-tipped glass needle or micropipette to deliver DNA into host cells. Transformation is the process by which foreign DNA is transferred […]
Difference Between pre-mRNA and mRNA
The key difference between pre-mRNA and mRNA is that pre-mRNA is the first product of the transcribed gene and contains both non-coding sequences (introns) and coding sequences (exons) while mRNA is the second product of a transcribed gene which contains only coding sequences. Gene is the functional unit of heredity. It is a specific DNA […]
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