HPLC vs Fast HPLC
The technique used for separating components of a mixture is called liquid chromatography. There are several techniques to achieve this purpose out of which HPLC is very popular. HPLC stands for High Performance Liquid Chromatography that identifies, quantifies and separates components of a liquid mixture in a fast and efficient manner. Fast HPLC is a special application of HPLC which has of late caught the attention of those involved in the study of solutions. This article will highlight the differences between HPLC and fast HPLC for the benefit of the readers.
HPLC makes use of high pressure to push solvents so that they pass through a column. It is an ideal way to separate and study various compounds such as amino acids, nucleic acids, carbohydrates, steroids, antibiotics and hydrocarbons. HPLC has many advantages over other methods of separation of compounds of mixtures such as higher resolution and faster analysis. It obliterates the need for repacking and regeneration and offers better control over parameters thus increasing the efficiency of separation. HPLC is ideally suited for large scale applications as it can separate very small particles and allows use of high pressures that makes it easy for solvents to flow easily.
Recently, research scientists at Schering Plough research institute developed a rapid HPLC that is an advanced version of HPLC as it allows for even faster separation of compounds from a mixture. Also known as fast HPLC, this technique makes use of a 2.7um fused core silica particles that are made by fusing 0.5um porous silica over a solid silica particle. These uniquely made particles make the procedure of separation very fast and that too at a low backpressure than what is used in HPLC.
Fast HPLC gets results comparable with UPLC but is being considered superior as it does not require very expensive ultra high pressure equipment. What is great is that fast HPLC does not require any new laboratory protocols.
HPLC vs Fast HPLC
• HPLC is a technique of liquid chromatography that has become very popular all over the world.
• Fast HPLC is a new version of HPLC developed by research scientists at Schering Plough Institute.
• Fast HPLC is HPLC that utilizes uniquely made silica particles
• Fast HPLC is better than even UPLC as it does not need ultra high pressure equipment
• Fast HPLC does not need any new lab protocols.